Join us for a free workshop covering fast protein liquid chromatography (FPLC) presented by Dr. Nodar Makharashvili from The University of Texas at Austin. FPLC is a valuable tool for an array of purification needs. It was originally developed for separating macromolecules at preparative scale and features high loading capacity, biocompatible aqueous buffer systems, fast flow rates, and availability of a large assortment of stationary phases (e.g., ion exchange, gel filtration, reversed phase, and affinity). The system makes reproducible separation possible by incorporating a high level of automation including autosamplers, gradient program control, and peak collection. It can be used to simply purify a protein of interest from a complex mix, isolate monoclonal antibodies from hybridomas, clear excess dyes from an antibody conjugation process, or even study the protein-protein interactions. In addition to proteins, the method is applicable to other kinds of biological samples including oligonucleotides and plasmids.
This will be a lab-based workshop and will cover topics surrounding the use of our GE AKTA Pure FPLC, including:
- FPLC layout components, and readouts
- Protein purification methods
- Benefits of automation in protein purification
- Creating, editing, and executing templates for rotuine protocols
10:30am -12:00pm – FPLC Workshop
12:00pm – 1:00pm – Networking Lunch
The workshop is free but requires registration. Space is limited. Lunch will be provided.
WHO SHOULD ATTEND
Researchers, scientists, postdocs, students, and anyone interested in incorporating FPLC into their workflow.
DATE AND TIME
Tue, November 7, 2017; 10:30 AM – 1:00 PM CST
ABOUT THE PRESENTER
Dr. Nodar Makharashvili is a scientist with extensive research experience in biochemistry, biophysics, and molecular biology. He has authored scientific publications, spoken at international conferences, collaborated with research groups at other universities and has initiated and led intradepartmental research projects. He has also mentored graduate, undergraduate, and high school students resulting in the receipt of awards, as well as publications in peer-reviewed scientific journals. He received his Ph. D. in Biochemistry from Saint Louis University School of Medicine; his MS and BS degrees in organic chemistry and chemistry, respectively, from Tbilisi State University (Republic of Georgia). Dr. Makharashvili currently holds a postdoctoral position at the University of Texas of Austin.