MLAB 2431: Immunohematology

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MLAB 2431: Immunohematology

Dates: Jan. 16 – May 12, 2024
Length of Course: 16 Weeks
Classroom Hours: 48 (hybrid)
Laboratory Hours: 64

Immunohematology is a specialized branch of laboratory medicine which involves the study of blood group antigens and antibodies. The processes of quality testing include quality control, basic laboratory techniques and safety. It involves the study of the principles, procedures, clinical significance and practice of a wide variety of procedures including: donor selection, component preparation and use, and techniques used to detect antigen/antibody reactions which may adversely affect a patient receiving a transfusion. The topics to be covered include: donor screening, preparation of components, antigens and antibodies of the ABO, Rh and other blood group systems, pretransfusion testing procedures, hemolytic disease of the fetus and newborn, neonatal and obstetrical transfusion practice, autoimmune hemolytic anemias and adverse affects of transfusion.

Course Objectives

Apply the principles of safety, quality assurance, and quality control in immunohematology.
Describe the blood collection process.
State the preparation method, use, storage requirements, and expiration of each blood component.
Evaluate specimen acceptability.
State the principle of each testing procedure performed in Immunohematology.
State the characteristics of the ABO, Rh, and other blood group systems.
List the methods used to identify the most commonly encountered blood group antibodies.
Describe the three types of hemolytic disease of the fetus and newborn (HDFN).
Describe the testing, antibody specificity, and treatment for HDFN.
List the adverse complications of blood transfusion.
State the methods for evaluating a positive direct antiglobulin test.
State the types of immune hemolytic anemias including results of serologic testing and compatibility testing which must be performed.
State the requirements for performing compatibility testing.
Describe the quality control performed in Immunohematology.
Explain the next steps when the quality controls are out of range in Immunohematology.
Describe the immune process as it relates to Immunohematology.
Describe the donor selection process and accurately determine donor eligibility when given results of donor screening tests.
Describe the preparation and the appropriate use of blood components by determining the component needed based on laboratory data.
Describe the characteristics of the antigens and antibodies of the ABO, Rh, and other blood group systems, applying the knowledge to sample testing and case study materials.
Perform blood bank testing in the resolution of antibody problems, HDFN, and transfusion reactions.
Evaluate blood bank laboratory test results.
Evaluate additional testing required for certain laboratory results of a patient.
Correlate test results with the patient’s condition.

Syllabus

Review the video on the MLAB 2431 Syllabus, sign the SOU, and email to the instructor.

Textbook

Howard, Paula R., Basic and Applied Concepts of Blood Banking and Transfusion Practices, Fifth Edition, 2020, Mosby. ISBN: 978-0323697392

Exams

NOTE: Throughout the semester, exams are on different days of the week. Please look closely at the dates the exams are scheduled. Thank you.

Exam 1 On Campus

Covers Lecture Units 1 & 2, Labs 1 & 2

Blood Collection
Blood/Blood Components
Donor History/Physical

Monday, 2/5/24

EVC-8:30am Room 9317

Monday, 2/5/24

RRC-3:30pm

Exam 2 In-house

Covers Lecture Units 3, 4 & 5 (no Labs)
Immunology/Complement
Principles of Sero Testing in BB
Genetics

Mon, 2/19/24
EVC – 8:30am
Room 9317

Mon, 2/19/24
RRC – 3:30pm

Exam 3 Online

A review will be online from Monday, 2/26, 8:30am to Friday, 3/1, 11:59pm. Students must make an 80 or better on this review to open the exam.

Covers Lecture Units 6 & 7, Labs 3 & 5
ABO/H Blood Groups
Rh Blood Group System
ABO/D Typing Lab
Rh Phenotyping Lab

Opens Thursday,
2/29/2024
7:00 am

Closes
Friday,
3/1/2024,
7:00 pm

Exam 4 On Campus

Two Part Exam

Clinical II – Dry Competency
Selection of Controls for Antigen Typing ONLINE:
Opens 4/8 @ 0800
Closes 4/11 @ 2359 REQUIRED

A review will be online from Wednesday, 4/10/2024, 0830 to Monday, 4/15/2024, 0830. Students must make an 80 or better on this review to open the exam.

Covers Lecture Units 8, 9 & 10, Labs 4, 6 & 7

Pretransfusion Testing
Other Blood Group Systems
ID Unexpected Antibodies
Type/Screen Lab
Crossmatch Lab
ID of Unexpected Abs

Exam 4 Part 2 will be open from Monday, 4/15/2024, 0700 to 4/17/2024, 1900.

Opens Monday
4/8 @ 0800

Monday, 4/15/2024,
EVC-8:30am

Room 9317

Closes Thursday
4/11 @ 2359

Monday, 4/15/2024,
RRC-3:30pm

Exam 5

Two Part Exam

Exam Review opens 4/16/2024 to 4/22/2024, @ 1500.

Covers Lecture Units 11, Labs 8 & 10

Neonatal/Obstetrical Transfusion Practice

DAT Lab
Rh Immune Globulin Lab

Exam 5, Part 2 online opens 4/24/2024 @ 0700 to 4/25/2024 @ 1900

EVC

Room 9317 8:30am

RRC 3:30pm

Exam 6 online

Open book

Two Part Exam

Exam 6 Review opens Tuesday, April 30, 2024, at 7:00am and closes May 3, at 7:00pm

Exam Part 1 and Part 2 Covers Lecture Units 12 & 13, Lab 11

Adverse Complications
Investigation of the Positive DAT and Immune Hemolysis

Delayed Transfusion Rxn Lab

Opens Thursday, 5/2/2024,
7:00 am

Closes
Friday, 5/3/2024,
7:00 pm

Comprehensive Blood Bank Final – May 6, 2024

EVC – 9:00am to 11:00am
RRC – 3:30pm to 5:30pm
This is a tentative date for final – On Campus

Schedule

Admission tickets and prelab quizzes are due by 8:30 a.m. of the class day.

1/22/2024

Laboratory 1/22/2024	No onsite laboratory this week Lab 1: Donor History Lab 2: Donor Physical These are due Friday, January 26, 2024.
Textbook	Chapter 13
Lecture	*Review of Syllabus* Unit 1: Blood Collection \| Objectives \| Notes Part 1 \| Part 2
Assignments	Prelab 1: Donor Interview and Physical Due 1/29/2024 @ 0830 Admission Ticket 1: Blood Collection Due 1/29/2024 @ 0830 Prelab 2 – ABO/D Typing Due 1/29/2024 @ 0830 Admission Ticket 2 Blood and Blood Components Due 1/29/2024 @ 0830 Laboratory study questions Due 1/26/2024 @ 2359

1/29/2024

Laboratory	Lab 3: ABO and D Typing
Textbook	Chapter 14
Lecture	Unit 2: Blood and Blood Components \| Objectives \| Notes
Assignments	Laboratory Exercise Due 1/29/2024 @ 2359 Laboratory Study Questions Due 2/2/2024 @ 2359 Admission Ticket 3 – Immunology Testing Due 2/5/2024 @ 0830

2/5/2024

Laboratory	Lab 3: ABO and D Typing (repeat) Print two Type/Screen Report Forms for class
Textbook	Chapters 2 and 3
Lecture	Unit 3: Immunology and Complement Objectives \| Notes Part 1 \| Part 2 Unit 4: Principles of Serological Testing in Immunohematology Objectives \| Notes
Assignments	Laboratory exercise ONLY Due 2/5/2024 @ 2359 Prelab 3 – Type and Screen Due 2/12/2024 @ 0830 Admission Ticket 4: Genetics and ABO/H Due 2/12/2024 @ 0830

2/12/2024

Laboratory	Lab 4: Type and Screen Print one Type/Screen Report Form for class
Textbook	Chapters 4 and 5
Lecture	Unit 5 Genetics \| Objectives \| Notes Unit 6 ABO and H Blood Groups Part One \| Objectives \| Notes Unit 6 ABO and H Blood Groups Part Two \| Notes Practice ABO Discrepancies
Assignments	Laboratory Exercise Due 2/12/2024 @ 2359 Laboratory Study Questions Due 2/16/2024 @ 2359 Prelab 4 – Rh phenotyping and review of ABO/D Due 2/19/2024 @ 0830 Admission Ticket 5 – Rh and Compatibility Due 2/19/2024 @ 0830

2/19/2024

Laboratory	Lab 5: Rh Phenotyping \| Rh Phenotyping Results Form
Textbook	Chapters 6
Lecture	Unit 7: Rh Blood Group System \| Objectives \| Notes Part One Part 2
Assignments	Laboratory Exercise Due 2/19/2024 @ 2359 Laboratory Study Questions Due 2/23/2024 @ 2359 Prelab 5 – Crossmatch/Automation Due 2/26/2024 @ 0830 Admission Ticket 6 – Pretransfusion Compatibility Due 2/26/2024 @ 0830

2/26/2024

Laboratory	REPEAT T/S x 2 Please bring two Type/Screen Report Forms
Textbook	Chapter 8, 9, and 10
Lecture	Unit 8 – Pretransfusion Compatibility Testing Objectives \| Notes Part 1 \| Part 2 \| Part 3
Assignments	See exam information regarding the exam review (Must be taken before exam) Laboratory Exercise Due 2/26/2024 @ 2359 Laboratory Study Questions Due 3/1/2024 @ 2359 Prelab 6 – Review of Crossmatch and Discrepancies Due 3/4/2024 @ 0830

3/4/2024

Laboratory	Lab 6: Crossmatch \| Type/Screen Report Form
Textbook	Chapter 7, 8, 9, and 10
Lecture	Unit 8 – Pretransfusion Compatibility Testing Objectives \| Notes Part 1 \| Part 2 \| Part 3
Assignments	Laboratory exercise ONLY Due 3/4/2024 @ 2359 Admission Ticket 7 – Automation Other Blood Groups Due 3/25/2024 @ 0830

Spring Break 3/11/2024 to 3/15/2024 Enjoy!

3/18/2024

Laboratory	Print two Type/Screen Report Forms for practice for today. You will perform the following: Type and Screen Type and Crossmatch for two units
Textbook	Chapter 7
Lecture	Unit 9: Other Blood Group Systems continued \| Objectives \| Notes Part 1 \| Part 2 Antibody Information Chart (upload under Assignments). Due 3/31/2024 @ 2359
Assignments	No study questions due on Friday Laboratory exercise ONLY Due 3/18/2024 @ 2359 Admission Ticket 9 Part 1 – Identification of Unexpected Antibodies Due 3/25/2024 @ 0830

3/25/2024

Laboratory	WET Laboratory Practical 1. You will perform the following procedures: Type and crossmatch for two units An ABO/D on an ER patient Print two Type/Screen Report Forms for the practical.
Textbook	Chapter 8
Lecture	Unit 10: Identification of Unexpected Antibodies \| Objectives \| Notes Bring the following pages with you to class today: Panel Studies for Discussion
Assignments	Laboratory practical will be collected at the end of the lab period. Prelab 7 Panel Studies \| Due 4/1/2024 @ 0830 Admission Ticket 10 Neonatal/OB Part 1 Due 4/1/2024 @ 0830

4/1/2024

Laboratory	Lab 7: Identification of Unexpected Antibodies Panels for SQ 7 Tutorial: Selecting Positive and Negative Controls for Testing Reagent Anti-serum in the Blood Bank
Textbook	Chapter 12
Lecture	Unit 11: Neonatal and Obstetrical Transfusion Practice Objectives \| Notes Part 1 \| Part 2 \| Part 3
Assignments	Laboratory Exercise Due 4/1/2024 @ 2359 Laboratory Study Questions Due 4/5/2024 @ 2359 Admission Ticket 11 Neonatal/OB Part 2 and 3 Due 4/8/2024 @ 0830 Prelab 9 DAT Compilation of Prelabs 1-9; This exercise is optional/ not graded

4/8/2024

Laboratory	Clinical II – Dry Competency Selection of Controls for Antigen Typing ONLINE: Opens 4/8 @ 0800 Closes 4/11 @ 2359 REQUIRED
Textbook	Chapter 12
Lecture	Unit 11: Neonatal and Obstetrical Transfusion Practice (continued) This is a Zoom meeting. EVC @ 10:am RRC @ 4:00pm
Assignments	(For Clinical II – Three panels – work on today in class and review with instructor – to be graded before leaving class) Prelab 8 DAT Due 4/15/2024 @ 0830 Prelab 10 Rh Immune Globulin Workup Due 4/15/2024 @ 0830 Admission Ticket 12 – Adverse Complications of Transfusions Due 4/22/2024 @ 0830

4/15/2024

	Lab 8: Direct Antiglobulin Test (DAT) Do not download the following documents in Google docs because the format will not be correct. Thank you. DAT laboratory result form DAT versus IAT (short animation) Lab 10: Rh Immune Globulin Workup Rh Immune Globulin Cord Blood Result Form Fetal Screen Form
Textbook	Chapter 11
Lecture	Unit 12: Adverse Complications of Blood Transfusion \| Objectives \| Notes Unit 13: Investigation of the Positive DAT and Immune Hemolysis \| Objectives \| Notes
Assignments	Laboratory Exercise Due 4/15/2024 @ 2359 Laboratory Study Questions Due 4/19/2024 @ 2359 Admission Ticket 13 Organ Transplantation and Quality Assurance Due 4/22/2024 @ 0830 Prelab compilation of Prelabs 7-11. This exercise is optional/not graded Prelab 11 Delayed Transfusion Reaction Workup Due 4/22/2024 @ 0830 Prelab 12 Pulling it all together Due 4/22/2024 @ 0830

4/22/2024

Laboratory	Clinical Panel Competency (Paper) in laboratory Lab 11: Delayed Transfusion Reaction Perform an elution panel
Textbook	Chapter 11
Lecture	Unit 14: Hemotherapy and Organ Transplantation Objectives \| Notes Unit 15: Quality Assurance in the Blood Bank Objectives \| Notes
Assignments	Laboratory Exercise Due 4/22/2024 @ 2359 Laboratory Study Questions Due 4/26/2024 @ 2359

4/29/2024

Laboratory	WET Practical Laboratory Instructor will provide paperwork Clinical II – Repeat Dry Panel Competency or N/A
Textbook	Chapter 16
Lecture	Review for final
Assignments	Practical paperwork due upon completion

5/6/2024

Laboratory	N/A
Textbook	N/A
Lecture	N/A
Final	See Exam Schedule

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