MLAB 2434: Clinical Microbiology

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Current Students

MLAB 2434: Clinical Microbiology

Dates: January 16 – May 12, 2024
Length of Course: 16 Weeks

Total Number of Hours: 128 (approximate)

Instruction in the theory, practical application, and pathogenesis of clinical microbiology, including collection, setup, identification, susceptibility testing, and reporting procedures. Laboratory exercises will endeavor to provide the student with the most comprehensive experiences possible, but will rely mainly on the commonly measured differential characteristics of select bacterial groups.

Course Objectives

By the end of this course the student should be able to:

Demonstrate proficiency in aseptic handling of bacteriologic specimens.
Demonstrate an understanding of bacterial, viral and rickettsial descriptions and classifications.
Choose the proper media for and correctly process bacteriologic specimens.
Identify unknown organisms using techniques presented in laboratory exercises.

Syllabus

Please print the MLAB 2434 Syllabus and bring it to class.

Textbook

Mahon, Connie R., and Lehman, Donald, Textbook of Diagnostic Microbiology, Seventh Edition, Elsevier, ISBN 9780323829977

Exams

Exam 1 (In Class)	Covers:Lectures:Bacterial Cell Structure (Chap. 1), Host-Pathogen Interaction( Chap. 2), Public Health (Chap. 3), Control of Microorganisms (Chap. 4), Performance Improvement (Chap. 5), Specimen Collection (Chap. 6), Microscopic Exam (Chap. 7), Colony Morphology (Chap. 8), Antibiotics (Chap. 12), Antibiotic Sensitivity Testing (Chap. 13), Bioterror (Chap. 30)Labs: Specimen Acceptability, Antibiotic Sensitivity, Streaking for Isolation, Prep of Smears & Gram Stain, and Clinical Specimen Preparation	EVC:Tuesday, Feb. 13, 20248:30 – 9:45 amRoom 9317	RRC:Tuesday, Feb. 13, 20243:30- 4:45 pmRoom 3121
Exam 2 (In Class)	Covers:Lectures- Staphylococci (Chap. 14), Streptococci (Chap. 15), Skin & Soft Tissue Infections (Chap. 33)Labs- Staphylococcus, Streptococci, previous labs	EVC:Tuesday, Feb. 27, 20248:30 – 9:30 amRoom 9317	RRC:Tuesday, Feb. 27, 20243:30- 4:30 pmRoom 3121
Exam 3 (In Class)	Covers:Lectures:Neisseria/Moraxella (Chap. 17), Haemophilus (Chap. 18), Respiratory Infections (Chap. 32), CNS Infections (Chap. 35), Bacteremia (Chap. 36), Genital Infections (Chap. 38)Labs- Neisseria, Haemophilus, Blood Culture, previous labs	EVC: Tuesday, March 26, 20248:30 – 9:45 amRoom 9317	RRC: Tuesday, March 26, 20243:30- 4:45 pmRoom 3121
Exam 4 (In Class)	Covers:Lectures-Enterics (Chap. 19), Vibrio etc. (Chap. 20), Non-fermenting GNRs (Chap. 21), GI Infections (Chap. 34), UTIs (Chap. 37)Labs- Enterics, Packaged GNR, Non-fermenters	EVC:Tuesday, April 23, 20248:30 – 9:45 amRoom 9317	RRC:Tuesday, April 23, 20243:30- 4:45 pmRoom 3121
Exam 5 (In Class)	Covers:Lectures- GPR (Chap. 16), Anaerobes (Chap. 22), Mycobacteria (Chap. 26)Labs- Anaerobes, Mycobacteria	EVC:Tuesday, April 30, 20248:30 – 9:30 amRoom 9317	RRC:Tuesday, April 30, 20243:30- 4:30 pmRoom 3121

Final Exam – Tuesday May 7, 2024

Covers all course material.

Eastview Class: Rm. 9317 8:30 am- 10:30 am

Round Rock Class: Rm 3121 3:30 p.m- 5:30 pm

Schedule

Disregard any topics/labs/assignments with strikeouts.

01/10/24

Textbook/Lecture Topics	Review Course syllabus Syllabus is found under “Syllabus” in Blackboard. Bring the syllabus signature sheet to the first class meeting. Due 1/16/2024
Laboratory	NA
Assignment	Complete Admission ticket: Performance Improvement. This can be found under “Assignments” in Blackboard. Due 1/16/2024 @ 8:30 am Complete Admission ticket: Control of Microorganisms. This can be found under “Assignments” in Blackboard. Due 1/16/2024 @ 8:30 am

01/16/24

Textbook/Lecture Topics	Course syllabus- Q & A Syllabus is found under “Syllabus” in Blackboard. Ch. 5, pp. 102-121: Performance Improvement \| Objectives \| Notes Ch. 4, pp. 65-100: Control of Microorganisms \| Objectives \| Notes Discussion of Microbe Projects Project Due 4/30/24
Laboratory	TBA
Assignment	Post in the DB the topic of your Microbe Project- faculty must approve topic. Due 1/21/2024

01/17/24

Textbook/Lecture Topics	Ch. 6, pp. 123-138: Specimen Collection & Handling \| Objectives \| Notes Ch. 7, pp. 139-154: Microscopic Examination \| Objectives \| Notes
Laboratory	Lab: Specimen Acceptability
Assignment	Specimen Acceptability Activity Review & Print “Specimen Site Descriptions & Acceptability. Found under Course Materials button. Use these reference documents to complete the activity. Go to Assignments button in Bb, select Lab Activities. Complete the quiz using the Specimen Site Descriptions & Acceptability packet. Time limit is 30 minutes. Due 1/18/24 @ 11:59 pm Complete Admission ticket: Cell Structure. This can be found under “Assignments” in Blackboard. Due 1/23/24 @ 8:30 am Pre-Lab: Prep of Smears and Gram Staining. This can be found under “Assignments” in Blackboard. Due 1/23/2024 @ 8:30 am

01/23/24

Textbook/Lecture Topics	Ch. 1, pp. 2-23: Bacterial Cell Structure, Physiology, Metabolism & Genetics \| Objectives \| Notes Review Specimen Acceptability Activity
Laboratory	Lab: Preparation of Smears and Gram Staining
Assignment	Complete Admission ticket: Host-Pathogen Interaction. This can be found under “Assignments” in Blackboard. Due 1/24/2024 @ 8:30 am Print the Gram Stain Log Sheet to record results. Bring to class EVERYDAY. This will be used during the entire semester.

01/24/24

Textbook/ Lecture Topics	Ch. 2, pp. 25-49: Host-Pathogen Interaction \| Objectives \| Notes
Laboratory	Lab: Clinical Specimen Preparation, Gram Staining, and Interpretation
Assignment	Pre-Lab: Streaking for Isolation. This can be found under “Assignments” in Blackboard. Due 1/30/24 by 8:30 am Watch the video on the streak plate method. (2 minutes) Watch the video on aseptic transfer. (3 minutes)

01/30/24

Textbook/Lecture Topics	Ch. 8, pp. 169-182: Colony Morphology \| Objectives \| Notes
Laboratory	Lab: Streaking for Isolation and Interpreting Primary Culture Result
Assignment	Complete Admission ticket: Public Health. This can be found under “Assignments” in Blackboard. Due 1/31/2024 @ 8:30 am

01/31/24

Textbook/Lecture Topics	Ch. 3, pp. 50-64: Public Health & The Microbiology Lab \| Objectives \| Notes Ch. 30, pp. 753-774: Bioterrorism \| Objectives \| Notes
Laboratory	Lab: Streaking for Isolation and Interpreting Primary Culture Result (cont.)
Assignment	Activity- Bioterrorism Solve the Outbreak Navigate to the Dropbox in BlackBoard (Bb) In the Misc. Folder- click on the Bioterrorism activity- instructions are in the Bb activity. Due 2/4/24 @ 11:59 pm Discussion Board Post: first read the article found in Bb under the discussion board. Then post a response as to whether Typhoid Mary was a villain or victim. Due 2/4/2024 by 11:59 p.m. View the History of Bioterrorism video ( ~ 30 minutes) Complete Admission ticket: Antimicrobials This can be found under “Assignments” in Blackboard. Due 2/6/2024 @ 8:30 am Pre-Lab: Sensitivity. This can be found under “Assignments” in Blackboard. Due 2/6/24 @ 8:30 am

02/06/24

Textbook/Lecture Topics	Ch. 12, pp. 250-269: Antibiotic Mechanisms of Action & Resistance \| Objectives \| Notes Ch. 13, pp. 271-305: Antimicrobial Susceptibility Testing \| Objectives \| Notes
Laboratory	Lab: Antibiotic Sensitivity testing
Assignment	TBD

02/07/24

Textbook/Lecture Topics	Exam #1 Review
Laboratory	Lab: Sensitivity testing (cont.)
Assignment	Review the discussion on Blood agar Plates and Hemolysis Protocols. Complete Admission ticket: Staph. This can be found under “Assignments” in Blackboard. Due 2/13/2024 @ 8:30 am. Pre-Lab: Staphylococcus. This can be found under “Assignments” in Blackboard. Due 2/13/2024 Watch coagulase test video. (5 minutes) Watch catalase test video. ( 2 minutes)

02/13/24

Textbook/Lecture Topics	Ch. 14, pp. 308-323: Staphylococci \| Objectives \| Notes Print the Aerobic Gram Positive Cocci Flowchart.
Laboratory	Lab: Staphylococcus
Assignment

02/14/24

Textbook/Lecture Topics	Review Exam #1
Laboratory	Lab: Staphylococcus (cont.)
Assignment	Complete Admission ticket: Streptococci. This can be found under “Assignments” in Blackboard. Due 2/20/24 @ 8:30 am Pre-Lab: Differentiation of Streptococcus. This can be found under “Assignments” in Blackboard. Due 2/20/24 @ 8:30 am Unknown #1 Preparation: Print documents listed under Unknown #1 under tomorrow’s lab.

02/20/24

Textbook/Lecture Topics	Ch. 15, pp. 324-346: Streptococci \| Objectives \| Notes
Laboratory	Lab: Streptococcus Discussion on Unknown Work-Ups Guidelines on How to Approach Unknown Workups Unknown Worksheet Instructions, Examples , and blank template Grading rubric Unknown #1 Instructions, Worksheet, and Rubric
Assignment	Case Study Assignment #1: This can be found under “Assignments” in Blackboard. Due 2/25/24 at 11: 59 p.m.

02/21/24

Textbook/Lecture Topics	Ch. 33, pp. 832-866: Skin & Soft Tissue Infections \| Objectives \| Notes Exam #2 Review
Laboratory	Strep Lab (cont.) Unknown #1 (cont.)
Assignment	Complete Admission ticket: Neisseria/Moraxella. This can be found under “Assignments” in Blackboard. Due 2/27/24 @ 8:30 am Pre-Lab: Neisseria and Moraxella catarrhalis. This can be found under “Assignments” in Blackboard. Due 2/27/2024 @ 8:30 am Unknown #2: Preparation. This can be found in Bb under “Assignments,” “Unknowns.” Please print all documents and bring to class.

02/27/24

Textbook/Lecture Topics	Ch. 17, pp. 371-389: Neisseria & Moraxella \| Objectives \| Notes
Laboratory	Lab: Neisseria Unknown #2
Assignment	Case Study Assignment #2. This can be found under “Assignments” in Blackboard. Due 3/3/24 at 11:59 p.m.

02/28/24

Textbook/Lecture Topics	Ch. 38, pp. 942-972: Genital Tract Infections \| Objectives \| Notes
Laboratory	Lab: Neisseria (cont.) Unknown #2 (cont.)
Assignment	Virtual Unknown #2: Urogenital #1. Complete the Urogenital Unknown under “Assignments” in Blackboard. Due 3/3/24 by 11:59 p.m.

03/05/24

Textbook/Lecture Topics	Ch. 36, pp. 904-921: Bacteremia \| Objectives \| Notes Clinically Significant Yeast
Laboratory	Lab: Blood culture
Assignment	TBD

03/06/24

Textbook/Lecture Topics	Ch. 35, pp. 889-903: CNS Infections \| Objectives \| Notes
Laboratory	Blood culture Lab (cont.)
Assignment	Complete Admission ticket: Haemophilus. This can be found under “Assignments” in Blackboard. Due 3/19/2024 @ 8:30 am Pre-Lab: Haemophilus. This can be found under “Assignments” in Blackboard. Due 3/19/2024 @ 8:30 am.

03/19/24

Textbook	Ch. 18, pp. 390-415: Haemophilis & Other Fastidious GNR \| Objectives \| Notes
Laboratory	Lab: Haemophilus
Assignment	TBD

03/20/24

Textbook	Ch. 32, pp. 791-829: Respiratory Infection \| Objectives \| Notes Exam 3 review
Laboratory	Lab: Haemophilus (cont.)
Assignment	Virtual Unknown #1: Respiratory. Complete the Respiratory Unknown under “Assignments” in Blackboard. Due 3/24/24 by 11:59 p.m.

03/26/24

Textbook	Ch. 29, pp. 708-751: Clinical Virology \| Objectives \| Notes Part 1\| Part 2
Laboratory	TBD
Assignment	Complete Admission ticket: Chlamydia/ Mycoplasma. This can be found under “Assignments” in Blackboard. Due 3/27/24 @ 8:30 am

03/27/24

Textbook/Lecture Topics	These are self-instructional units- do NOT come to campus today. Ch. 24, pp. 543-557: Chlamydia & Rickettsia \| Objectives \| Notes Ch. 25, pp. 558-568: Mycoplasma & Ureaplasma \| Objectives \| Notes Review Exam #3
Laboratory
Assignment	Pre-Lab: Non-Fermenters GNR. This can be found under “Assignments” in Blackboard. Due 4/02/24 @ 8:30 am

04/02/24

Textbook/Lecture Topics	Ch. 21, pp. 474-494: NF GNB and Miscellaneous GNR \| Objectives \| Notes
Laboratory	Lab: Non-Fermentative Gram-Negative Bacilli
Assignment	Complete Admission ticket: Vibrio, Aeromonas. This can be found under “Assignments” in Blackboard. Due 4/03/24 @ 8:30 am

04/03/24

Textbook/Lecture Topics	Ch. 20, pp. 455-472: Vibrio, Aeromonas, Campylobacter, and Helicobacter \| Objectives \| Notes
Laboratory	Lab: Non-Fermentative Gram-Negative Bacilli (cont.)
Assignment	Complete Admission ticket: Enterobacteracae. This can be found under “Assignments” in Blackboard. Due 4/9/24 @8:30 am Pre-Lab #8: Enterobacteriaceae “Enterics.” This can be found under “Assignments” in Blackboard. Due 4/9/2024 @ 8:30 am Pre-Lab #9: GNR ID. This can be found under “Assignments” in Blackboard. Due 4/9/24 @ 8:30 am Case Study Assignment #3. This can be found under “Assignments” in Blackboard. Due 4/7/24 by 11: 59 p.m.

04/09/24

Textbook/Lecture Topics	Ch. 19, pp. 417-453: Enterobacteriaceae \| Objectives \| Notes
Laboratory	Lab: Identification of Enterics
Assignment	Case Study Assignment #4. This can be found under “Assignments” in Blackboard. Due 4/14/24 at 11:59 p.m.

04/10/24

Textbook/Lecture Topics	Ch. 37, pp. 922-940: Urinary Tract Infections \| Objectives \| Notes
Laboratory	Lab: Packaged Microbial ID System
Assignment

04/15/24

Textbook/Lecture Topics
Laboratory	Unknown: Urine Culture
Assignment

04/16/24

Textbook/Lecture Topics

Ch. 34, pp. 869-888: GI Infections & Food Poisoning | Objectives| Notes

Laboratory

Unknown: Urine Culture

Microscan: Autoscan Procedure

Assignment

Complete Admission ticket: Gram Positive Rod. This can be found under “Assignments” in Blackboard. Due 4/17/24 @ 8:30 am
Virtual Unknown #3: Stool. Complete the Stool Unknown under “Assignments” in Blackboard. Due 4/21/24 by 11:59 p.m.
Check out this video.

04/17/24

Textbook/Lecture Topics	Ch. 16, pp. 496-530: Aerobic Gram-Positive Rods \| Objectives \| Notes Part 1 \| Part 2 \| Part 3 \| Part 4 GPR Flowchart
Laboratory	Unknown: Urine Culture (con’t)
Assignment	Pre-Lab: Anaerobes. This can be found under “Assignments” in Blackboard. Due 4/23/24 @ 8:30 am

04/23/24

Textbook/Lecture Topics	Ch. 22, pp. 496-531: Anaerobes \| Objectives \| Notes Part 1 \| Part 2
Laboratory	Lab: Anaerobes
Assignment	NA

04/24/24

Textbook	Ch. 26, pp. 570-595: M. tuberculosis & other mycobacteria \| Objectives \| Notes
Laboratory	Lab: Anaerobes (cont.) Lab: Mycobacteria
Assignment	Case Study Assignment #5. This can be found under “Assignments” in Blackboard. Due 4/28/24 at 11:59 p.m.

04/30/24

Textbook/Lecture Topics	Exam #5 Presentation of Microbe Projects
Laboratory
Assignment

05/01/24

Textbook/Lecture Topics
Laboratory
Assignment

00/00

Textbook/Lecture Topics	~~Benchmark “B”~~ ~~Lab 4: Determination of Methicillin Resistance (cont.)~~ ~~Lab 4: Determination of Methicillin Resistance (CHROMagar)~~ ~~Slide Review Exercise #1: Mixed Sources~~ ~~Unknown #3~~ Unknown #3 Preparation. This can be found in Bb under “Assignments,” “Unknowns.” Please print all documents and bring to class. Unknown #4 Preparation. This can be found in Bb under “Assignments,” “Unknowns.” Please print all documents and bring to class. ~~Unknown #4 (cont.)~~ ~~Lab 11: Anaerobes (virtual)~~ ~~Unknown #4~~ ~~Unknown #3 (cont.)~~ ~~Gram Stain Benchmark: Slide set “A”~~ ~~Lab 9: Packaged GNR ID system~~ Unknown: Tissue Culture
Laboratory
Assignment

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MLAB 2434: Clinical Microbiology

Current Students

MLAB 2434: Clinical Microbiology

Course Objectives

Syllabus

Textbook

Exams

Final Exam – Tuesday May 7, 2024

Schedule

Disregard any topics/labs/assignments with strikeouts.

01/10/24

01/16/24

01/17/24

01/23/24

01/24/24

01/30/24

01/31/24

02/06/24

02/07/24

02/13/24

02/14/24

02/20/24

02/21/24

02/27/24

02/28/24

03/05/24

03/06/24

03/19/24

03/20/24

03/26/24

03/27/24

04/02/24

04/03/24

04/09/24

04/10/24

04/15/24

04/16/24

04/17/24

04/23/24

04/24/24

04/30/24

05/01/24

00/00

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2024 Health Sciences Spring Completion Ceremonies

ACC Health Sciences Regional Simulation Center

ACC and AFD Celebrate Five Years of Red Angels Partnership

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